Device
Part:BBa_I766218:Design
Designed by: Nili Sommovilla Group: iGEM07_UCSF (2007-10-22)
HMTM1-EE under pGAl1 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 209
Illegal EcoRI site found at 1713
Illegal EcoRI site found at 2411
Illegal XbaI site found at 1366
Illegal PstI site found at 2762 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 209
Illegal EcoRI site found at 1713
Illegal EcoRI site found at 2411
Illegal PstI site found at 2762 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 209
Illegal EcoRI site found at 1713
Illegal EcoRI site found at 2411
Illegal BglII site found at 1129
Illegal BamHI site found at 3057
Illegal XhoI site found at 1026
Illegal XhoI site found at 1110
Illegal XhoI site found at 1312
Illegal XhoI site found at 2496 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 209
Illegal EcoRI site found at 1713
Illegal EcoRI site found at 2411
Illegal XbaI site found at 1366
Illegal PstI site found at 2762 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 209
Illegal EcoRI site found at 1713
Illegal EcoRI site found at 2411
Illegal XbaI site found at 1366
Illegal PstI site found at 2762
Illegal AgeI site found at 633 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
For more information concerning our cloning approach and construct design, see the documentation in the UCSF2007 iGEM wiki.
Source
Composite Device